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mouse primary antibody against human gapdh  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse primary antibody against human gapdh
    Antioxidant effect of Zn on HUC-MSCs <t>through</t> <t>Nrf2/Sirt3</t> signaling pathway. A The effect of Zn on the level of ROS production. DCFH-DA staining was used to measure intracellular ROS production. The scale bar indicates 100 μm. B Over time, under zinc treatment, the expression of Nrf2, Sirt3, and PGC-1α protein was measured by an illustrative Western blot. Four independent experiments were carried out and typical results were given. C, D Normalized the quantification of Nrf2 ( C ) and PGC-1α ( D ) to <t>GAPDH</t> and measured by image J. The results are expressed as mean ± S.D., * p < 0.05, ** p < 0.01, *** p < 0.001. E Real-time quantitative PCR results of Nrf2, Sirt3 and PGC-1α gene expression under Zn treatment ( n = 5). * p < 0.05, ** p < 0.01
    Mouse Primary Antibody Against Human Gapdh, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 20663 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse primary antibody against human gapdh/product/Santa Cruz Biotechnology
    Average 97 stars, based on 20663 article reviews
    mouse primary antibody against human gapdh - by Bioz Stars, 2026-02
    97/100 stars

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    1) Product Images from "Anti-oxidative effect of zinc in human umbilical cord mesenchymal stem cells"

    Article Title: Anti-oxidative effect of zinc in human umbilical cord mesenchymal stem cells

    Journal: Biophysics Reports

    doi: 10.52601/bpr.2021.200046

    Antioxidant effect of Zn on HUC-MSCs through Nrf2/Sirt3 signaling pathway. A The effect of Zn on the level of ROS production. DCFH-DA staining was used to measure intracellular ROS production. The scale bar indicates 100 μm. B Over time, under zinc treatment, the expression of Nrf2, Sirt3, and PGC-1α protein was measured by an illustrative Western blot. Four independent experiments were carried out and typical results were given. C, D Normalized the quantification of Nrf2 ( C ) and PGC-1α ( D ) to GAPDH and measured by image J. The results are expressed as mean ± S.D., * p < 0.05, ** p < 0.01, *** p < 0.001. E Real-time quantitative PCR results of Nrf2, Sirt3 and PGC-1α gene expression under Zn treatment ( n = 5). * p < 0.05, ** p < 0.01
    Figure Legend Snippet: Antioxidant effect of Zn on HUC-MSCs through Nrf2/Sirt3 signaling pathway. A The effect of Zn on the level of ROS production. DCFH-DA staining was used to measure intracellular ROS production. The scale bar indicates 100 μm. B Over time, under zinc treatment, the expression of Nrf2, Sirt3, and PGC-1α protein was measured by an illustrative Western blot. Four independent experiments were carried out and typical results were given. C, D Normalized the quantification of Nrf2 ( C ) and PGC-1α ( D ) to GAPDH and measured by image J. The results are expressed as mean ± S.D., * p < 0.05, ** p < 0.01, *** p < 0.001. E Real-time quantitative PCR results of Nrf2, Sirt3 and PGC-1α gene expression under Zn treatment ( n = 5). * p < 0.05, ** p < 0.01

    Techniques Used: Staining, Expressing, Western Blot, Real-time Polymerase Chain Reaction, Gene Expression

    Zinc regulated Nrf2 expression in HUC-MSCs. A Illustrative western blotting measured Nrf2, PGC-1α and SIRT3 protein expression. Four independent experiments were implemented and the typical results are presented. B, C, D Quantification of Nrf2 ( B ), PGC-1α ( C ) and SIRT3 ( D ) were normalized to GAPDH and measured by Image J. Results are presented as mean ± S.D. * p < 0.05, ** p < 0.01. E Immunofluorescent staining of Nrf2 (red) in HUC-MSCs. DAPI was used for nucleus staining (blue). The scale bar indicates 50 μm
    Figure Legend Snippet: Zinc regulated Nrf2 expression in HUC-MSCs. A Illustrative western blotting measured Nrf2, PGC-1α and SIRT3 protein expression. Four independent experiments were implemented and the typical results are presented. B, C, D Quantification of Nrf2 ( B ), PGC-1α ( C ) and SIRT3 ( D ) were normalized to GAPDH and measured by Image J. Results are presented as mean ± S.D. * p < 0.05, ** p < 0.01. E Immunofluorescent staining of Nrf2 (red) in HUC-MSCs. DAPI was used for nucleus staining (blue). The scale bar indicates 50 μm

    Techniques Used: Expressing, Western Blot, Staining



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    Santa Cruz Biotechnology mouse primary antibody against human gapdh
    Antioxidant effect of Zn on HUC-MSCs <t>through</t> <t>Nrf2/Sirt3</t> signaling pathway. A The effect of Zn on the level of ROS production. DCFH-DA staining was used to measure intracellular ROS production. The scale bar indicates 100 μm. B Over time, under zinc treatment, the expression of Nrf2, Sirt3, and PGC-1α protein was measured by an illustrative Western blot. Four independent experiments were carried out and typical results were given. C, D Normalized the quantification of Nrf2 ( C ) and PGC-1α ( D ) to <t>GAPDH</t> and measured by image J. The results are expressed as mean ± S.D., * p < 0.05, ** p < 0.01, *** p < 0.001. E Real-time quantitative PCR results of Nrf2, Sirt3 and PGC-1α gene expression under Zn treatment ( n = 5). * p < 0.05, ** p < 0.01
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    Antioxidant effect of Zn on HUC-MSCs <t>through</t> <t>Nrf2/Sirt3</t> signaling pathway. A The effect of Zn on the level of ROS production. DCFH-DA staining was used to measure intracellular ROS production. The scale bar indicates 100 μm. B Over time, under zinc treatment, the expression of Nrf2, Sirt3, and PGC-1α protein was measured by an illustrative Western blot. Four independent experiments were carried out and typical results were given. C, D Normalized the quantification of Nrf2 ( C ) and PGC-1α ( D ) to <t>GAPDH</t> and measured by image J. The results are expressed as mean ± S.D., * p < 0.05, ** p < 0.01, *** p < 0.001. E Real-time quantitative PCR results of Nrf2, Sirt3 and PGC-1α gene expression under Zn treatment ( n = 5). * p < 0.05, ** p < 0.01
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    Antioxidant effect of Zn on HUC-MSCs <t>through</t> <t>Nrf2/Sirt3</t> signaling pathway. A The effect of Zn on the level of ROS production. DCFH-DA staining was used to measure intracellular ROS production. The scale bar indicates 100 μm. B Over time, under zinc treatment, the expression of Nrf2, Sirt3, and PGC-1α protein was measured by an illustrative Western blot. Four independent experiments were carried out and typical results were given. C, D Normalized the quantification of Nrf2 ( C ) and PGC-1α ( D ) to <t>GAPDH</t> and measured by image J. The results are expressed as mean ± S.D., * p < 0.05, ** p < 0.01, *** p < 0.001. E Real-time quantitative PCR results of Nrf2, Sirt3 and PGC-1α gene expression under Zn treatment ( n = 5). * p < 0.05, ** p < 0.01
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    Image Search Results


    Antioxidant effect of Zn on HUC-MSCs through Nrf2/Sirt3 signaling pathway. A The effect of Zn on the level of ROS production. DCFH-DA staining was used to measure intracellular ROS production. The scale bar indicates 100 μm. B Over time, under zinc treatment, the expression of Nrf2, Sirt3, and PGC-1α protein was measured by an illustrative Western blot. Four independent experiments were carried out and typical results were given. C, D Normalized the quantification of Nrf2 ( C ) and PGC-1α ( D ) to GAPDH and measured by image J. The results are expressed as mean ± S.D., * p < 0.05, ** p < 0.01, *** p < 0.001. E Real-time quantitative PCR results of Nrf2, Sirt3 and PGC-1α gene expression under Zn treatment ( n = 5). * p < 0.05, ** p < 0.01

    Journal: Biophysics Reports

    Article Title: Anti-oxidative effect of zinc in human umbilical cord mesenchymal stem cells

    doi: 10.52601/bpr.2021.200046

    Figure Lengend Snippet: Antioxidant effect of Zn on HUC-MSCs through Nrf2/Sirt3 signaling pathway. A The effect of Zn on the level of ROS production. DCFH-DA staining was used to measure intracellular ROS production. The scale bar indicates 100 μm. B Over time, under zinc treatment, the expression of Nrf2, Sirt3, and PGC-1α protein was measured by an illustrative Western blot. Four independent experiments were carried out and typical results were given. C, D Normalized the quantification of Nrf2 ( C ) and PGC-1α ( D ) to GAPDH and measured by image J. The results are expressed as mean ± S.D., * p < 0.05, ** p < 0.01, *** p < 0.001. E Real-time quantitative PCR results of Nrf2, Sirt3 and PGC-1α gene expression under Zn treatment ( n = 5). * p < 0.05, ** p < 0.01

    Article Snippet: Mouse primary antibody against human GAPDH (Santa Cruz, sc-166574; 37kD; 1:2000), rabbit anti-human Nrf2 (Abcam, 100kD; 1:1000), rabbit anti-human Sirt3 (Abcam, 35kD; 1:1000) and rabbit anti-human PGC-1α (Abcam, 100kD; 1:1000) were used.

    Techniques: Staining, Expressing, Western Blot, Real-time Polymerase Chain Reaction, Gene Expression

    Zinc regulated Nrf2 expression in HUC-MSCs. A Illustrative western blotting measured Nrf2, PGC-1α and SIRT3 protein expression. Four independent experiments were implemented and the typical results are presented. B, C, D Quantification of Nrf2 ( B ), PGC-1α ( C ) and SIRT3 ( D ) were normalized to GAPDH and measured by Image J. Results are presented as mean ± S.D. * p < 0.05, ** p < 0.01. E Immunofluorescent staining of Nrf2 (red) in HUC-MSCs. DAPI was used for nucleus staining (blue). The scale bar indicates 50 μm

    Journal: Biophysics Reports

    Article Title: Anti-oxidative effect of zinc in human umbilical cord mesenchymal stem cells

    doi: 10.52601/bpr.2021.200046

    Figure Lengend Snippet: Zinc regulated Nrf2 expression in HUC-MSCs. A Illustrative western blotting measured Nrf2, PGC-1α and SIRT3 protein expression. Four independent experiments were implemented and the typical results are presented. B, C, D Quantification of Nrf2 ( B ), PGC-1α ( C ) and SIRT3 ( D ) were normalized to GAPDH and measured by Image J. Results are presented as mean ± S.D. * p < 0.05, ** p < 0.01. E Immunofluorescent staining of Nrf2 (red) in HUC-MSCs. DAPI was used for nucleus staining (blue). The scale bar indicates 50 μm

    Article Snippet: Mouse primary antibody against human GAPDH (Santa Cruz, sc-166574; 37kD; 1:2000), rabbit anti-human Nrf2 (Abcam, 100kD; 1:1000), rabbit anti-human Sirt3 (Abcam, 35kD; 1:1000) and rabbit anti-human PGC-1α (Abcam, 100kD; 1:1000) were used.

    Techniques: Expressing, Western Blot, Staining